1 Center for Quantitative Biology, Peking University, Beijing, 100871,China 2 School of Mathematical Sciences, Peking University, Beijing,100871, China 3 School of Life Science, Peking University,Beijing, 100871,China * These authors contributed equally to this work. Rice Microarray Gene Expression Raw Data
1. Data Set 1:
The rice embryos were obtained from the seeds soaked in water for
2-3 days. The third and mature expanded leaves were collected when
they had just fully expended. Rice seedlings were harvested when
they were 2-3cm in height. The panicles were collected when they
were 0.6-4cm in length. Total RNA was isolated separately from rice
embryos, the third mature leaves, the fifth mature leaves, seedlings
and panicles with TRIzol Reagent from Invitrogen Life Technologies.
To obtain microarray data covering almost all genes in rice, total
RNA from different samples described above was mixed into a big pool
called Pre-amplified mRNA sample (PAM) by almost equal magnitude.
Eight subgroups were separated from the purified PAM. The half of
them were amplified and labeled by the One-Cycle Eukaryotic Target
Labeling Assay from Affymetrix (Santa Clara, CA, USA). The resulting
samples were called One-Cycle cRNA samples (OCS). The other half
were amplified and labeled by the Two-Cycle Eukaryotic Target
Labeling Assay from Affymetrix (Santa Clara, CA, USA). The resulting
samples were called Two-Cycle cRNA samples (TCS).
Data Set 1 (35.3M bytes)
2. Data Set 2:
This data set contained microarray data from
two different samples, including the third leaf primordium and the
third mature leaves of rice. Total RNA was extracted from the two
samples. And then each of them was separately amplified by two
methods, the One-Cycle Labeling Assay and the Two-Cycle Labeling
Assay (Affymetrix, Santa Clara, CA, USA). Two replicates were
applied for each Assay. Totally there are 8 hybridization samples
for the following microarray experiments.
3. Data Set 3:
This data set included 21 slides of Affymetrix
microarray. There are 7 different stages from rice, including the
third leaf primordium, the third mature leaves, and stamen samples
at stage 2, 3, 4, 5 and 6 (Stages are partitioned by the development
of Stamen). Biological replicates are
applied 3 times for each of them. All the 21 mRNA samples are
amplified with Two-Cycle Labeling Assay (Affymetrix, Santa Clara,
CA, USA).
All experimental procedures strictly followed instructions specified in the
Affymetrix GeneChip Expression Analysis Technical Manual.
Data Set 3 (84.3M bytes)