Engineering synthetic gene circuits for sensing, information processing and actuation in mammalian cells
主 题: Engineering synthetic gene circuits for sensing, information processing and actuation in mammalian cells
报告人: Dr. Zhen Xie（Tsinghua university） Prof.Liping Zhu（SUFE）
时 间: 2014-05-15 14:00-15:00
地 点: 理科1号楼1114（统计中心活动）
Synthetic gene circuits are designed to implement desired cellular functions in living cells through sensing, integration and processing of molecular information, which will have profound implications in basic biological research and biomedical applications. In the first part of my talk, I will introduce a design framework of orthogonal and reversible transcription activator-like effector repressors (TALERs) that bind newly designed hybrid promoters and exert transcriptional repression through steric hindrance of key transcriptional initiation elements. I will show that using the input-output transfer curves of individual TALERs enables accurate prediction of the behavior of modularly assembled TALER cascade and switch circuits. Then, I will present that TALER switches employing feedback regulation exhibit improved accuracy for microRNA-based cancer cell classification. In the second part of my talk, I will show our results of model-guided quantitative analysis of microRNA-mediated regulations on competing RNAs by using a synthetic gene circuit. Our computational and experimental results reveal that the relative abundance of miRNAs and competing RNAs, the binding free energies and the number of miRNA regulatory elements (MREs) have strong impacts on miRNA-mediated regulations on competing RNAs.